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June 06, 2007
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Determination of Nucleosides in NucleomaxX
NucleomaxX is a food supplement drink mix purporting to consist of a sugar cane (Saccharum officinarum) extract containing a high content (17%) of nucleosides as uridine called Mitocnol. NucleomaxX has been investigated as a source of uridine supplementation for the prevention and treatment of side effects related to the use of various nucleoside reverse transcriptase inhibitors (NRTI) in the treatment of HIV associated with mitochondrial toxicity. In support of a National Center for Complementary and Alternative Medicine (NCCAM) funded clinical study, the University of California San Francisco Medical School contracted TBAR to develop and validate a method to quantify nucleosides in NucleomaxX product. In addition to uridine, the prominent nucleoside was found to be a 2',3',5'-tri-O-acetyluridine (TAU). These two nucleosides were found to be the only nucleosides present in analytically significant quantities. The figure above is a chromatogram of a NucleomaxX sample preparation. The small peak at about 3.1 minutes is uridine, and the large peak at about 7.8 minutes is the TAU. The large peak at 2 minutes is ascorbic acid, which is added to the sample to prevent decomposition of the TAU. The identity of the TAU was verified by Flora Research Laboratories and the BCIT Natural Health Products Research Group, and was revealed in a presentation at the International Meeting of HIV Lipodystrophy that was held in Sydney, Australia in July, 2007. For more information regarding the testing of NucleomaxX contact Tampa Bay Analytical Research. |
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